UCSF Laboratory for Cell Analysis (Diller Comprehensive Cancer Center Cytometry Core)

The Laboratory for Cell Analysis provides cytometric, microscopic, and genomic support and services for the UCSF Helen Diller Family Comprehensive Cancer Center. The LCA was established in 1983 with the following responsibilities:

  • Provide flow and image cytometers and the technical support needed to make use of these instruments.
  • Educate UCSF students, staff, and faculty in cytometry technology and applications.
  • Develop new cytometric methods and new cytometric applications.

The LCA currently occupies two sites within the UCSF community with cytometry and microscopy services offered at the Mission Bay Campus and genome wet-lab services focused in the Cancer Research Building (CRB) at the Mt. Zion campus. The LCA is supporting over 17 instruments representing a long-term capital investment of over $7M.

The LCA was one of the first large cytometry cores in the country and has developed a broad repertoire of instrumentation, technical expertise, and a thoughtful infrastructure to help address a wide variety of experimental demands and novel applications in many model systems. From apoptosis to zymogen assays in organisms from adipocytes to zebrafish, the LCA has accumulated working protocols and a broad theoretical knowledge base to aid Cancer Center investigators.

Ben Braun
braunb@peds.ucsf.edu

Core Lab Website:
http://cancer.ucsf.edu/cores/lca

ServiceStandard CostJCCC/CFAR CostExternal Cost
Flow Cytometry - Analyzer   
Flow Cytometry - Cell Sorting   
Microscopy - Confocal Spinning   
Microscopy - Confocal Spinning - off peak   
Microscopy - Laser Microdissection   
Microscopy - Basic/Digital   
Instrument Operation/Training   
Analysis Workstation   
Bioanalyzer - Instrument Time   
Nucleofector   
Nanodrop 8000   
QPCR (Syber, TaqMan, High Throughput) Instrument Time   
QS3D dPCR - Instrument Time   
Technician Time   
Additional Reagents